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. 2016 Mar 3;291(18):9733–9747. doi: 10.1074/jbc.M115.687806

FIGURE 2.

FIGURE 2.

DETA-NO preconditioning promotes hCSC anti-apoptotic effect. A, representative FACS analysis with annexin V/PI staining showing H2O2-induced apoptosis in both preconditioned (PC) (250 μm DETA-NO for 12 h) and vehicle-treated groups. B, quantitative data analysis for A. C, examination of antiapoptotic gene expression at the mRNA level in response to DETA-NO preconditioning. D, representative images of Western blot showing the dose-dependent up-regulation of antiapoptotic gene expression in response to DETA-NO preconditioning. E, representative images of Western blot showing the time-related up-regulation of antiapoptotic gene expression with 250 μm DETA-NO preconditioning. F, representative images of Western blot showing antiapoptotic gene expression levels after DETA-NO withdrawal over 24 h. The quantitative data for Western blots shown in D–F are provided in supplemental Fig. 4. Values are means ± S.D. Error bars represent S.D. * indicates p < 0.05 versus control; n = 3 independent experiments. RC, recovery.