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. 2016 Mar 3;291(18):9733–9747. doi: 10.1074/jbc.M115.687806

FIGURE 3.

FIGURE 3.

DETA-NO preconditioning did not alter the capacities of hCSC cardiac gene expression, cell proliferation and proliferation. A, quantitative PCR of cardiac gene expressions in hCSCs pretreated with vehicle or DETA-NO. cTNT, cardiac troponin T. B, BrdU incorporation assay examined by flow cytometry. hCSCs were preconditioned with or without DETA-NO for 12 h, and cells were then cultured in growth medium supplemented with 10 μm BrdU for 3 days. C, MTT assay. hCSCs were preconditioned with vehicle or DETA-NO for 12 h, and cells were then cultured in growth medium 3 days. D and E, hCSCs were preconditioned (PC) with vehicle or DETA-NO, and cells were then cultured in differentiation medium for 3 weeks (wks). The expression level of Troponin I was examined by quantitative PCR (D). Expression of MG53 protein was examined by Western blotting (E). Data are shown as mean ± S.D. from three independent experiments. Error bars represent S.D.