FIGURE 5.
DETA-NO preconditioning elevates phosphorylation of ERK, AKT, STAT3, and NFκB-p65 in hCSCs. A, representative images of Western blot showing the activation of ERK, AKT, STAT3, and NFκB-p65 phosphorylation in a dose-dependent manner in response to DETA-NO preconditioning. B, representative images of Western blot showing the time-dependent up-regulation of ERK, AKT, STAT3, and NFκB-p65 phosphorylation with 250 μm DETA-NO preconditioning (PC). C, representative images of Western blot showing phosphorylation levels of ERK, AKT, STAT3, and NFκB-p65 after DETA-NO withdrawal over 24 h. The quantitative data for Western blots shown in A–C are provided in supplemental Fig. 5. Experiments were performed at least three times (n = 3 independent experiments). t, total; RC, recovery.
