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. 2016 Mar 3;291(18):9733–9747. doi: 10.1074/jbc.M115.687806

FIGURE 7.

FIGURE 7.

Knockdown of STAT3 and NFκB-p65 expression diminishes the NO-mediated cytoprotective effect in hCSCs. A, schematic protocol to describe procedures for lentiviral infection and the subsequent experiments. B, representative images and quantitative analysis of Western blots (WB) confirming the efficiency of knockdown of STAT3 and NFκB-p65 expression by shRNA. C, hCSCs expressing scrambled (Scr), STAT3, or NFκB-p65 shRNA were preconditioned with vehicle or DETA-NO and then challenged with H2O2. Oxidative stress-induced cytotoxicity was evaluated by LDH release assay. Data are presented as mean ratio of H2O2-induced LDH release to total LDH release in cells with S.D. D, representative FACS analysis with annexin V/PI staining showing H2O2-induced apoptosis in both DETA-NO-treated (250 μm for 12 h) and vehicle-treated hCSCs expressing scrambled, STAT3, or NFκB-p65 shRNA. E, quantitative data for early apoptosis induced by H2O2 shown in D. Values are means ± S.D. Error bars represent S.D. *, p < 0.05; **, p < 0.01, and ***, p < 0.001 versus the indicated control (Ctrl) group.