FIGURE 6.

Mutagenesis of key residues in the Wzt-C putative binding pocket alter O-antigen export activity. Size distribution of LPS species is altered by the mutation of candidate O-PS-binding site residues in R. terrigena ATCC 33257 Wzt-C. Samples were proteinase K-treated whole-cell lysates that were subject to SDS-PAGE and silver staining. A and B are representative silver-stained SDS-PAGE and an α-O12 immunoblot of proteinase K-treated samples, respectively. C is an α-Wzt-C_O12 Western blot that demonstrates expression of Wzt mutants.