FIGURE 2.

Both antibodies detect Fpr3 and do not recognize other Fpr family members. A, immunostaining of HEK293T cells to test the cross-reactivity of Fpr3-ECL1 (upper panel) and Fpr3-ECL2 (lower panel) for the indicated receptors. Insets show stainings with an anti-rhodopsin-epitope (Rho) antibody as a positive control for expression of the indicated receptors. Scale bars, 20 μm. B, average percentage of stained cells from three independent experiments. Numbers in parentheses denote positive versus total cells. Error bars, S.D. C, representative scatter plots of staining intensities for both antibodies in relative fluorescence units (RFU). Each dot represents an individual cell. Threshold (blue line) was set to maximal intensity level observed for mock-transfected cells. D, antibody dilution experiment examining the sensitivity of both antibodies tested in decreasing concentrations. Signals were normalized to 1:100 dilution. Immunoreactivity was compared with mock-transfected cells. Error bars, S.D.