FIGURE 5.

Prediction of K⁺ binding sites in Fet3p. Fet3p (PDB code 1ZPU) residues predicted to be involved in K⁺ binding are shown in ball and stick representation. Carbon atoms are colored in pink, nitrogen atoms are in dark blue, and oxygen atoms are in red. Bound copper atoms are represented as light blue spheres. The figures were generated using UCSF Chimera software. A, four predicted K⁺ sites, containing Glu-487, Asn-49, Asp-501, and Ser-161/Glu-662, are indicated in the catalytic domain of Fet3p. The K⁺ coordination sites containing Glu-487 (B), Asn-49 (C), or Ser-161/Glu-662 (D) are enlarged. The solid gray lines (D) indicate hydrogen bond networks.