FIGURE 7.

Iron deficiency up-regulated KHA1 expression. A, WT control cells cultured with (+) and without (−) iron (Fe) chelator BPS in the SC media were subjected to quantitation of KHA1 mRNA. B, three predicted Aft1p and Aft2p response elements at the promoter (within −700 bp). GGGTG core sequences are indicated along with one 5′ and two 3′ nucleotides. C, schematic presentation of lacZ reporter of KHA1 with site-directed mutations of AC (−158 and −157) to TG. D, The indicated strains expressing KHA1-lacZ reporter were cultured with or without BPS iron chelator and subjected to β-galactosidase assays. The average ± S.D. (n = 4) is presented. Single (*) and double (**) asterisks indicate p < 0.05 and p < 0.01, respectively, relative to the results of the same strain cultured in control media (no iron chelator). The pound symbol (#) indicates p < 0.05 relative to WT strain cultured in the same media.