TABLE 1.
Minimal inhibitory concentrations (μg/ml) of cells expressing the NorM-NG variants
R6G, rhodamine 6G; ET, ethidium; Cys-less, cysteine-less NorM-NG; Cu, copper phenanthroline.
| Variants | R6G | R6G + Cu | R6G + Cu + DTT | ET | ET + Cu | ET + Cu + DTT |
|---|---|---|---|---|---|---|
| pET15b | 3.0 | 3.0 | 3.0 | 0.5 | 0.5 | 0.5 |
| NorM-NG | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| Cys-less | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| D41A | 3.0 | 3.0 | 3.0 | 0.5 | 0.5 | 0.5 |
| T42C/L289C | 6.0 | 3.0 | 6.0 | 2.0 | 0.5 | 2.0 |
| A45C/V285C | 6.0 | 3.0 | 6.0 | 2.0 | 0.5 | 2.0 |
| A57C/Q284C | 6.0 | 3.0 | 6.0 | 2.0 | 0.5 | 2.0 |
| L58C/V269C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| L58C/F270C | 6.0 | 3.0 | 6.0 | 2.0 | 0.5 | 2.0 |
| A62C/F265C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| A62C/S266C | 6.0 | 3.0 | 6.0 | 2.0 | 0.5 | 2.0 |
| T42C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| L289C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| A45C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| V285C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| A57C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| Q284C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| L58C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| V269C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| F270C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| A62C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| F265C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |
| S266C | 6.0 | 6.0 | 6.0 | 2.0 | 2.0 | 2.0 |