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. 2016 Apr 29;7:563. doi: 10.3389/fmicb.2016.00563

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Copyright © 2016 Marlow, Skennerton, Li, Chourey, Hettich, Pan and Orphan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A schematic diagram of the proteomic SIP sampling approach used in this study. Sediment was collected from active seep areas at Hydrate Ridge North for incubation #5133 and Hydrate Ridge South for #3731 (see text for full sampling details). Sediment from both sample sites was used as inoculum in parallel methane-infused incubations, with one bottle from each set receiving 1 mM ¹⁴NH₄Cl and the other receiving 1 mM ¹⁵NH₄Cl. Both #3731 incubations were sampled for proteomic SIP after 17 and 326 days; both #5133 incubations were sampled for proteomic SIP after 160 days.