Table 1.

Details of the constituents that comprise the metagenomic database used in this study.

IN-HOUSE METAGENOMES
Metagenome name	Source material	Sequencing platform	# of ORFs
BC	Bead Capture from Hydrate Ridge Seep Sediment, E3A PC47, 0–9 cm	454	25208
V01	Hydrate Ridge Seep Sediment, AD 4635 PC16, 0–6 cm	Illumina	4599
V02	Hydrate Ridge Seep Sediment, E3A PC47, 0–9 cm	Illumina	4841
3730	Hydrate Ridge Seep Sediment, AD 4635 PC16, 0–6 cm	Illumina	118384
5133	Hydrate Ridge Seep Sediment, E3A PC47, 0–9 cm	Illumina	78724
5579	Hydrate Ridge Seep Sediment, E3A PC41, 0–12 cm	Illumina	133690
3datasets	Combined assembly of 3730, 5133, 5579		133255
5datasets	Combined assembly of 3730, 5133, 5579, VO1, VO2		246534
OTHER SEQUENCE DATA
Name	Source material	Sequencing platform	# of ORFs
Hallam Fosmidsa	Eel River Basin Seep Sediment	Sanger	2219
Meyerdierks Fosmidsb	Black Sea Microbial Mats	Sanger	3122
ANME-2ac	Capt Aryutinov Mud Volcano	Illumina	4031
ANME-2dd	Sediment/Wastewater Sludge Enrichment	Illumina	3427
Cultured Organisms	NCBI Genome Repository		591976

References correspond to

^aHallam et al. (2004).

^bMeyerdierks et al., 2010.

^cWang et al., 2014.

^dHaroon et al., 2013.

The list of cultured organisms whose genomes were included in the metagenome can be found in Supplemental Data Sheet 2.