Table 4.
15N enrichment values of proteome-derived peptides and bulk protein extract.
| Sample ID | Incubation Time (d) | Peptide LC MS/MS Analysis (SE) | Bulk Protein Extract nanoSIMS Analysis | |
|---|---|---|---|---|
| 15N Atom % Mean | % of Enriched Peptides (>5% 15N) | 15N Atom % Mean (SE) | ||
| #3731 14N T17d | 17 | 0.5 (0.03) | 0.44 (0.03) | 0.38 (0) |
| #3731 15N T17d | 17 | 0.4 (0.01) | 0.52 (0.07) | 0.45 (5x10-5) |
| #3731 14N T326d | 326 | 0.4 (0.09) | 0.62 (0.07) | 0.38 (0) |
| #3731 15N T326d | 326 | 0.8 (0.04) | 1.48 (0.04) | 0.53 (5x10-5) |
| #5133 14N T160d | 160 | 0.5 (< 0.01) | 0.87 (0.03) | NA |
| #5133 15N T160d | 160 | 9.5 (18.34) | 18.06 (0.68) | NA |