Table 3.
OTUs with largest and smallest log2 fold RNA:DNA ratios (Δ) in FL and PA fractions.
| Phylum/Class (OTU) | Order | Family | Genus (Tribe) | FL | PA | ||
|---|---|---|---|---|---|---|---|
| % | Δ (P) | % | Δ (P) | ||||
| HIGH RIBOSOMAL RNA:DNA RATIO | |||||||
| Alphaproteobacteria (133) | Rhodospirillales | Acetobacteraceae | – | 0.191 | 4.2 (***) | 0.046 | 2.87 (*) |
| Alphaproteobacteria (30) | Caulobacterales | Hyphomonadaceae | Hirschia | 0.148 | 3.21 (***) | 3.877 | 3.54 (***) |
| Betaproteobacteria (139) | Burkholderiales | Oxalobacteraceae | – | 0.024 | 2.74 (*) | 0.167 | 0.96 () |
| Betaproteobacteria (78) | Burkholderiales | Burkholderiaceae | – | 0.341 | 1.63 () | 0.166 | 1.65 (*) |
| Gammaproteobacteria (55) | Methylococcales | Methylococcaceae | Methylobacter | 0.408 | 3.36 (*) | 0.360 | 1.92 () |
| Deltaproteobacteria (41) | Bdellovibrionales | Bdellovibronaceae | OM27 | 0.066 | 4.36 (***) | 1.494 | 3.65 (***) |
| Sphingobacteria (65) | Sphingobacteriales | – | – | 0.077 | 3.71 (***) | 0.889 | 2.51 (***) |
| Cytophagia (90) | Cytophagales | – | – | 0.112 | 2.54 (***) | 1.453 | 2.06 () |
| Flavobacteria (235) | Flavobacteriales | bacV | – | 0.078 | 2 (***) | 0.162 | 0.92 () |
| Sphingobacteria (25) | Sphingobacteriales | bacVI | – | 0.085 | 3.77 (***) | 1.544 | 2.67 () |
| Sphingobacteria (72) | Sphingobacteriales | bacVI | – | 0.071 | 3.65 (***) | 0.502 | 2.47 (*) |
| Cyanobacteria (39) | Subsection I | Family I | Microcystis | 0.003 | 4.91 (***) | 0.821 | 2.8 (*) |
| Verrucomicrobia (49) | Opitutales | Opitutaceae | – | 0.319 | 1.63 (*) | 0.160 | −0.11 () |
| Planctomycetes (34) | Phycisphaerales | Phycisphaeraceae | CL500-3 | 0.567 | 2.55 (***) | 1.923 | 1.52 () |
| LOW RIBOSOMAL RNA:DNA Ratio | |||||||
| Alphaproteobacteria (9) | Rickettsiales | alfV | alfV-A (LD12) | 5.176 | −1.45 (**) | 0.283 | −2.6 (***) |
| Betaproteobacteria (33) | Methylophilales | betIV | betIV-A (LD28) | 2.512 | −0.05 () | 0.274 | −1.62 (*) |
| Flavobacteria (16) | Flavobacteriales | bacV | – | 0.616 | −0.49 () | 1.599 | −1.53 (*) |
| Sphingobacteria (291) | Sphingobacteriales | bacVI | – | 0.100 | −0.25 () | 0.024 | −2.18 (*) |
| Actinobacteria (318) | Acidimicrobiales | acIV | acIV-A (Iluma-A2) | 0.173 | −5.6 (***) | 0.045 | −5.03 (***) |
| Planctomycetes (359) | Planctomycetales | Planctomycetaceae | – | 0.038 | −2.22 () | 0.123 | −3.49 (***) |
| Chloroflexi (765) | SL56 marine group | – | – | 0.248 | −1.39 () | 0.046 | −3.78 (***) |
| Verrucomicrobia (26) | Opitutales | Opitutaceae | – | 1.025 | 0.04 () | 0.357 | −2.26 (*) |
| Gemmatimonadetes (145) | Gemmatimonadales | Gemmatimonadaceae | Gemmatimonas | 0.063 | −2.68 (***) | 0.141 | −3.94 (***) |
Top OTUs were included for each phylum and Proteobacteria and Bacteroidetes class that contained OTUs among the top 200 most abundant taxa that were differentially represented in RNA and DNA data. We also reported the overall relative abundance of the OTUs across all FL or PA datasets (%) and the adjusted P-values reported by DEseq2 [ < 0.01 (*), < 0.001 (**), < 0.0001 (***)].