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RP-HPLC of oxidative folding of linear MCoTI-II(C4A). Reduced peptide was dissolved in 0.1 M ammonium bicarbonate (pH 8.2) and quenched after 1 min with 10% TFA. A well-resolved peak, corresponding to a peptide with two disulfide bonds formed (IIa), eluted earlier than the reduced peptide. Time points taken at 1 and 20 min are shown. The axes are for the 1-min data. The 20-min data has been shifted and scaled by 0.5 for clarity. To see this figure in color, go online.
