Table 1.

Photophysical Properties of Various Azaindole and Azatryptophan Analogs in Neutral Water and L-Asparaginases

	λabs (nm)	λem (nm)	λmon (nm)a	Τ (ns)b
7-Azaindole	288	386		0.91
(7-aza)Trp	289	400		0.65
2,7-Diazaindole	295	335	320	0.22
		370	400	10.10
		495	550	0.21[rise], 1.30
(2,7-aza)Trp	300	340	320	0.26
		380	380	0.27 (12%), 10.07 (88%)
		500	540	0.26[rise], 0.58
AnsA	280	318	320	2.71 (16%), 6.34 (86%)
(7-aza)Trp68-AnsA	300	370	380	2.4 (65%), 10.9 (35%)
(2,7-aza)Trp68-AnsA	300	335	330	0.24 (59%), 1.34 (41%)
		500	520	0.25[rise], 1.73
(2,7-aza)Trp68-AnsA+Asp	300	335	330	0.26 (73%), 1.34 (27%)
		500	520	0.25[rise], 1.73
AnsB	280	315	320	2.4 (15%), 4.6 (85%)
(7-aza)Trp66-AnsB	300	368	380	2.3 (75%), 10.8 (25%)
(2,7-aza)Trp66-AnsB	300	335	330	1.67
(2,7-aza)Trp66-AnsB+Asp	300	335	330	1.46

^aλ_mon is the wavelength at which the measurement of relaxation dynamics was monitored.

^bValues are ±0.03 ns in uncertainty. Values in parentheses indicate percentages and brackets denote the rise component.