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. 2016 Apr 29;7:153. doi: 10.3389/fphys.2016.00153

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Copyright © 2016 Salvaggio, Marino, Albano, Pecoraro, Camiolo, Tibullo, Bramanti, Lombardo, Saccone, Mazzei and Brundo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fold change of zinc concentrations in zebrafish embryos (mean ± SD). The samples contaminated with low concentrations of ZnCl₂ 25 mg/L, do not present differences with the control samples (untreated); athigher concentrations (50 and 150 mg/L of ZnCl₂) the Zn levels was increased after 7 days of contamination (p < 0.0001) respect to control (p < 0.0001). Values are expressed as a mean ± SD of four independent experiments performed in triplicate. Statistically significant differences by two-way analysis of variance (ANOVA) were performed.