Figure 3.

Efflux Gene Expression in Total and Persister Cells of Wild-Type E. coli Measured by RNA-seq and qPCR

(A) Heatmap showing relative transcript abundance of persistence related genes in total cells and persister cells. Scale below the heatmap indicates log2-normalized transcript abundance relative to the mean expression level (T, total cells; P, persister cells). See also Table S1.

(B) Scheme of the TolC related efflux system complexes, the number of cylinders indicating the copy number of the protein in each efflux complex.

(C) Scheme of the tolC operon.

(D) Relative gene expression level in total cells, persister cells, and regrown cells of wild-type E. coli measured by qPCR. The bars indicate mean of at least three independent experiments; error bar indicates SD.