Figure 3. Western blot evaluation of selected proteins.

(a) Western blot showing statistically significant different levels (P < 0.05) of four proteins (HSP90B1, LMNA, ACTN1 and ALDOC) in Ob-hA-MSCs vs Co-hA-MSCs. In the figure are reported the cropped gels/blots obtained by each protein evaluation. All gels were run in the same experimental conditions (see material and methods for details). (Full-length blots of each tested protein are reported in Supplementary Figure S2). (b) Quantification of the expression of the tested four proteins. The optical density of each sample was measured and normalized using a GAPDH run on the same gel. The data are expressed as percent relative expression, i.e. for each protein, the sample with the highest expression of that protein was set at 100%. The bottom and top of each box represent the 25th and 75th percentile, respectively; the thick band within each box shows the 50th percentile (the median). The ends of the whiskers represent the minimum and maximum values of each group of data.