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. 2016 Apr 29;6:25270. doi: 10.1038/srep25270

Table 1. Identification and characterization of proteins differently expressed in Obese hA-MSCs vs Non-obese hA-MSCs.

Spot no.a DIGE Obese/Non-obese hAMSCsb DIGE (P-value)c Protein name Gene Symbold MW (Da)e PIf Gene IDg Protein codeh
3114 1.96 0.029 Annexin A5 ANXA5 35936 4.93 308 P08758
873 1.86 0.024 Alpha-actinin-1 ACTN1 103057 5.25 87 P12814
2710 1.77 0.031 Serpin B6 SERPINB6 42621 5.18 5269 P35237
2748 1.77 0.031 Annexin A1 ANXA1 38714 6.57 301 P04083
3711 1.66 0.0037 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCHL1 24824 5.33 7345 P09936
1536 1.63 0.026 Myosin-9 MYH9 226532 5.50 4627 P35579
      LIM domain and actin-binding protein 1 LIMA1 85225 6.41 51474 Q9UHB6
489 1.59 0.022 Filamin-A FLNA 280739 5.70 2316 P21333
      Filamin-B FLNB 278164 5.47 2317 O75369
662 1.49 0.00067 Heat shock protein 105 kDa HSPH1 96864 5.27 10808 Q92598
3335 1.49 0.041 Prohibitin PHB 29804 5.57 5245 P35232
660 1.47 0.0099 Programmed cell death 6-interacting protein PDCD6IP 96023 6.13 10015 Q8WUM4
2879 1.47 0,0042 Elongation factor 1-delta EEF1D 31121 4.90 1936 P29692
2730 1.47 0.049 Heterogeneous nuclear ribonucleoprotein A1 HNRNPA1 38746 9.17 3178 P09651
522 1.44 0.0018 Protein phosphatase 1 regulatory subunit 12A PPP1R12A 115281 5.31 4659 O14974
1823 1.41 0.036 Dihydropyrimidinase-related protein 3 DPYSL3 61963 6.04 1809 Q14195
      Prelamin-A/C LMNA 74139 6.57 4000 P02545
      T-complex protein 1 subunit zeta CCT6A 58024 6.24 908 P40227
2813 1.40 0.022 Annexin A2 ANXA2 38604 7.57 302 P07355
      LIM and SH3 domain protein 1 LASP1 29717 6.61 3927 Q14847
521 1.39 0.0012 Protein phosphatase 1 regulatory subunit 12A PPP1R12A 115281 5.31 4659 O14974
1047 1.38 0.0056 Myosin-9 MYH9 226532 5.50 4627 P35579
      Transitional endoplasmic reticulum ATPase VCP 89265 5.14 7415 P55072
492 1.37 0.0056 Filamin-A FLNA 280739 5.70 2316 P21333
      Filamin-B FLNB 278164 5.47 2317 O75369
2925 1.37 0.053 Annexin A2 ANXA2 38604 7.57 302 P07355
      LIM and SH3 domain protein 1 LASP1 29717 6.61 3927 Q14847
574 1.33 0.040 Ribosome-binding protein 1 RRBP1 152472 8.69 6238 Q9P2E9
1996 1.32 0.049 Vimentin VIM 53651 5.05 7431 P08670
452 1.28 0.0017 Nodal modulator 1 NOMO1 134324 5.54 23420 Q15155
918 1.27 6.4e–005 Ubiquitin-like modifier-activating enzyme 1 UBA1 117849 5.49 7317 P22314
      Alpha-actinin-1 ACTN1 103057 5.25 87 P12814
      Alanine-tRNA ligase, mitochondrial AARS2 107340 5.87 57505 Q5JTZ9
1395 1.27 0.0018 ATP-dependent 6-phosphofructokinase, platelet type PFKP 85596 7.50 5214 Q01813
      Protein transport protein Sec23A SEC23A 86160 6.64 10484 Q15436
3408 1.26 0.0013 40S ribosomal protein SA RPSA 32854 4.79 3921 P08865
1866 1.26 0.022 Pyruvate kinase PKM PKM 57937 7.96 5315 P14618
2861 1.26 0.049 Annexin A2 ANXA2 38604 7.57 302 P07355
2955 1.25 0.038 LIM and SH3 domain protein 1 LASP1 29717 6.61 3927 Q14847
1476 1.23 0.0029 Stress-70 protein, mitochondrial HSPA9 73680 5.87 3313 P38646
      Annexin A6 ANXA6 75873 5.41 309 P08133
604 1.23 0.0032 ATP-citrate syntase ACLY 120839 6.95 47 P53396
      Leucine—tRNA ligase,cytoplasmic LARS 134466 6.95 51520 Q9P2J5
279 1.23 0.014 Pyruvate kinase PKM PKM 57937 7.96 5315 P14618
903 1.22 0.0011 Staphylococcal nuclease domain-containing protein 1 SND1 101996 6.74 27044 Q7KZF4
2769 1.22 0.016 Eukaryotic translation initiation factor 3, subunit I EIF3I 36501 5.38 8668 Q13347
456 1.21 0.0005 Leucine-rich PPR motif-containing protein, mitochondrial LRPRRC 157905 5.81 10128 P42704
1874 1.21 0.018 Pyruvate kinase PKM PKM 57937 7.96 5315 P14618
1236 1.20 0.0079 LIM domainand actin-binding protein 1 LIMA1 85225 6.41 51474 Q9UHB6
1017 1.20 0.019 Programmed cell death 6-interacting protein PDCD6IP 96023 6.13 10015 Q8WUM4
      Hexokinase-1 HK1 102485 6.36 3098 P19367
2770 −6.13 0.041 Annexin A1 ANXA1 38714 6.57 301 P04083
3162 −3.34 0.041 Chloride intracellular channel protein 1 CLIC1 26922 5.09 1192 O00299
3248 −2.21 0.051 S-methyl-5′-thioadenosine phosphorylase MTAP 31236 6.75 4507 Q13126
1385 −2.05 0.019 78 kDa glucose-regulated protein HSPA5 (Grp78) 72332 5.07 3309 P11021
3412 −1.93 0.0030 Heat shock protein beta-1 HSPB1 (HSP27) 22782 5.98 3315 P04792
3452 −1.70 0.00024 Heat shock protein beta-1 HSPB1 (HSP27) 22782 5.98 3315 P04792
3794 −1.59 0.031 Chain A, Three-Dimensional Structure Of Class Pi Glutathione S-Transferase From Human Placenta (Glutathione S-transferase P) GSTP1 23355 5.43 2950 P09211
2345 −1.55 0.048 6-phosphogluconate dehydrogenase, decarboxylating PGD 53139 6.8 5226 P52209
2606 −1.42 0.029 Fructose-bisphosphate aldolase C ALDOC 39455 6.41 230 P09972
      Mitochondrial import receptor subunit TOM40 homolog TOMM40 37893 6.79 10452 O96008
2326 −1.41 0.0093 Serpin H1 SERPINH1 46440 8.75 871 P50454
798 −1.39 0.046 Lon protease homolog, mitochondrial LONP1 106489 6.01 9361 P36776
1243 −1.37 0.0038 Ran GTPase-activating protein 1 RANGAP1 63541 4.63 5905 P46060
2090 −1.36 0.041 Alpha-aminoadipic semialdehyde dehydrogenase ALDH7A1 58487 8.21 501 P49419
      RuvB-like 1 RUVBL1 50228 6.02 8607 Q9Y265
2125 −1.35 0.017 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial DLST 48755 9.10 1743 P36957
1245 −1.35 0.023 Caldesmon CALD1 93231 5.62 800 Q05682
1310 −1.31 0.035 Protein disulfide-isomerase A4 PDIA4 72932 4.96 9601 P13667
      Acylamino-acid-relasing enzyme APEH 81224 5.29 327 P13798
829 −1.30 0.028 Endoplasmin HSP90B1 (Grp94) 92468 4.76 7184 P14625
1408 −1.28 0.00018 79 kDa glucose-regulated protein HSPA5 (Grp78) 72332 5.07 3309 P11022
2258 −1.26 0.028 Alpha-enolase ENO1 47168 7.01 2023 P06733
      6-phosphogluconate dehydrogenase, decarboxylating PGD 53139 6.8 5226 P52209
1327 −1.23 0.014 Protein disulfide-isomerase A4 PDIA4 72932 4.96 9601 P13667
      Prolyl endopeptidase PREP 80699 5.53 5550 P48147
2621 −1.23 0.049 Actin, cytoplasmic 2 ACTG1 41792 5.31 71 P63261
      COP9 signalosome complex subunit 4 COPS4 46268 5.57 51138 Q9BT78
1786 −1.22 0.0028 60 kDa heat shock protein, mitochondrial HSPD1 (HSP60) 61054 5.70 3329 P10809
1377 −1.21 0.049 78 kDa glucose-regulated protein HSPA5 (Grp78) 72332 5.07 3309 P11021
      Heat shock cognate 71 kDa protein HSPA8 70898 5.37 3312 P11142

aSpot numbering to indicate the positions of spots in preparative gel.

bAverage abundance ratio (Obese/Non-obese hA-MSCs) as calculated by DeCyder Analysis.

cP-value at Student’s t test.

dGene symbol from NCBI.

eTheoretical molecular weight (Da).

fTheoretical pI.

gGene ID from NCBI.

hProtein code from Swiss Prot.