Figure 2.

Pseudocobalamin Poorly Supports Growth of B₁₂-Dependent Eukaryotic Algae

(A–H) Growth yield (OD₇₃₀) of algae in liquid medium supplemented with or without cyanocobalamin or cyanopseudocobalamin (at 0.07, 0.7, or 7 nM) in batch culture after at least two transfers (until the cells had died in the −B₁₂ treatment). (A) O. tauri, (B) A. carterae, (C) P. lutheri, (D) T. pseudonana, (E) A. anophagefferens, (F) L. rostrata, (G) E. gracilis, and (H) C. reinhardtii B₁₂-dependent metE mutants are shown [29]. Cbl, cyanocobalamin; PsCbl, cyanopseudocobalamin. ^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001 compared with the equivalent concentration of Cbl (two-tailed Student’s t test; mean ± SEM; n = 3). See also Figures S3 and S4.