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. 2016 Mar 9;2(4):242–252. doi: 10.1021/acscentsci.6b00019

Figure 3.

Figure 3

Results from kinetic analysis of inhibitor candidates with the hCBS truncated dimer. (a) Lineweaver–Burk plot for hCBS inhibition with 6S, detecting H2S production in the presence Pb(OAc)2. (b) Secondary-plot of bis-α-hydrazino acid (6S) inhibition of H2S production by hCBS yielding a Ki value of 48 ± 2 μM. (c) UV/vis spectroscopic profile of model PLP-inhibitor adducts formed nonenzymatically. Conditions: 0.26 mM hydrazino acid 6S, 0.2 mM PLP, 50 mM KPO4 (pH 8.0), scans every 10 min, isosbestic point = 355 nm, t1/2 = 7 min (left spectrum); conditions: 0.33 mM aminooxy acid 9S, 0.2 mM PLP, 50 mM KPO4 (pH 8.0), scans recorded every 10 min, isosbestic point = 360 nm, t1/2 = 15 min (right spectrum).