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. 2016 Mar 9;2(4):242–252. doi: 10.1021/acscentsci.6b00019

Figure 4.

Figure 4

Reversal of enhanced cell death in CBS-overexpressing (CBSOE) SH-SY5Y cells exposed to high CBS substrates by CBS inhibitor 6S. (a) Fluorescence (right) and phase contrast (left) micrographs of lentiviral vector transduced SH-SY5Y CBSOE cells. Fluorescence indicates CBS-EGFP (reporter gene) expression. (b) Western blot results confirming that CBS was markedly expressed compared to nontransduced control (C) cells. Only the full length CBS (63 kDa) was detected but not the truncated CBS (45 kDa). (c) Inhibition by CBS inhibitor (CBSI) 6S of H2S production in SH-SY5Y CBSOE cell homogenates in the presence of l-cysteine (1 mM) and l-homocysteine (0.1 mM). Data are presented as mean ± SEM, n = 3. ANOVA: F (2, 6) = 421.247, p < 0.05; ***p < 0.001 against no inhibitor control by Bonferroni. (d) CBS inhibition by 6S reversed the enhanced cell death in CBSOE cells subjected to OGD (24 h) in the presence of high substrates. Cell viability is expressed as fraction to control without OGD (not shown). ANOVA for high substrate conditions: F (3, 11) = 10.248, p < 0.05; **p < 0.01 against without 6S by Bonferroni. Data are mean ± SEM, n = 3–4.