Neuroprotection by CBS inhibitor 6S against ischemic
injury induced by transient middle cerebral artery occlusion (tMCAO).
(a) Inhibition of H2S synthesizing activity in brain homogenate
at varying concentrations of CBSI 6S. Data are presented as mean ±
SEM, n = 3–7; **p < 0.01,
***p < 0.001 against no inhibitor control (C)
by Bonferroni. (b) The right MCA was occluded by the insertion of
a suture through the carotid artery for 100 min. The blood flow in
the affected area was monitored by a Laser-Doppler device showing
a reduction of about 70–75% and returned to pre-MCAO levels
upon reperfusion, n = 7–10. (c) Infarct volumes
at 24 h after tMCAO. Top panel shows representative TTC-stained forebrain
sections of a vehicle-treated, a CBSI 6S-pretreated and
a CBSI 6S-post-treated rat. CBSI 6S or vehicle
was administered by intracerebroventricular (icv) injection 30 min
before tMCAO (CBSI pre-30 min) or 1 h after middle cerebral artery
occlusion (CBSI post 1 h). (d) Bottom panel shows the calculated infarct
volumes of the three groups, n = 5–10, ANOVA:
F (2, 19) = 7.89, *p < 0.05, **p < 0.01 against vehicle control group by Bonferroni.