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. Author manuscript; available in PMC: 2016 Apr 29.
Published in final edited form as: Nature. 2015 Sep 23;526(7571):126–130. doi: 10.1038/nature15250

Figure 3. HSCs localize adjacent to sinusoids but distant from arterioles and transition zone vessels in tibias.

Figure 3

a–c, Distance to the nearest arteriole (a), sinusoid (b), or transition zone vessel (c). d–f, Percentages of all HSCs and random spots within 10μm of an arteriole (d), sinusoid (e), or transition zone vessel (f). g, Percentages of HSCs and random spots that were closest to arterioles, sinusoids, or transition zone vessels. h–j, Representative images of α-catulin-GFP+c-kit+ HSCs (arrows) that localized immediately adjacent to an arteriole (h), a sinusoid (i), or a transition zone vessel (j). All data reflect mean±s.d. from bone marrow plugs (390–550 μm thick) from the diaphysis of 6 tibias. A total of 2977 HSCs were analyzed in 6 independent experiments. In panels a–c the statistical significance was assessed by Kolmogorov–Smirnov analysis. Statistical significance in panels d–g was assessed by Student’s t-tests (*, P<0.05; **, P<0.01; ***, P<0.001).