Figure 1.

Effect of a CB1 receptor agonist on leptin-induced reactive oxygen species formation in primary hypothalamic neurons. Dihydrorhodamine-loaded primary hypothalamic neurons isolated from postnatal day 1 C57BL/6J mice were treated with serum-free medium in the absence (b) or presence (c) of 0.5 μM arachidonylchloro ethanolamide with (d) or without (c) 1 μM AM251. After 30 min, the cells were exposed to 100 ng ml⁻¹ leptin for an additional 30 min and then analysed with a fluorescence microscope. A representative micrograph of control cells is shown in (a). Scale bar: 20 m.