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. 2016 Apr 29;14:129. doi: 10.1186/s12957-016-0891-8

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© Zhang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Verification of RPS15A silencing in U251 cells after shRPS15A infection. a, b Infection efficiency was assessed by counting GFP-positive cells with fluorescence microscopy. c, d The mRNA levels of RPS15A in U251 cells with three treatments (shCon, shRPS15A-1, and shRPS15A-2) measured by qPCR analysis. The experiment was performed in triplicate and repeated three times. e The protein levels of RPS15A in U251 cells with three treatments (shCon, shRPS15A-1, and shRPS15A-2) determined by Western blot analysis. ***p < 0.001