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. 2016 Apr 29;17:8. doi: 10.1186/s12865-016-0146-z

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© Nian et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Thirty clones were chosen randomly from the single-chain variable fragment (scFv) library and the positive clones inserted into full scFv genes were identified by polymerase chain reaction (PCR). Lane M: 2000 bp DNA Marker (TaKaRa, JP). Lanes 1–30: amplified scFvs from different clones randomly picked. b PCR products of scFvs were digested by BstN I. 2000 bp DNA Marker (TaKaRa, JP). Lane M: 2000 bp DNA Marker (TaKaRa, JP). Lanes 1–30: Fingerprints of scFvs digested by BstN I