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. 2016 Apr 29;17:8. doi: 10.1186/s12865-016-0146-z

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© Nian et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — a Constructed scFv-Fcs (S78, S128, S117, S182) were amplified and their sizes were ~1.5 kb. Lane M: 2000 bp DNA Marker (TaKaRa, JP). b Expression level of scFv-Fcs (S78, S128, S117, S182) expressed at 25 °C and 32 °C, respectively, were tested by enzyme-linked immunosorbent assay