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. 2016 Apr 28;13:95. doi: 10.1186/s12974-016-0560-4

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© Moretti et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — 3D confocal analysis of double-labeled microglia and vessels after pMCAo and PBS and/or sildenafil (0.5 mg/kg) treatment. a Co-localization of M1 (COX-2 in red) and M2 (MRC-1 in green) markers in the leptomeningeal membranes, 72 h after ischemia. b MRC-1 (in red) is present in the perivascular microglia (in green). c Co-localization of M1 (COX-2 in green) and M2 (Arg-1 in red) markers in the leptomeningeal membranes 8 days after pMCAo, in a PBS-treated animal. Scale bar represents 10 μm (a–c)