Fig. 9.

Enrichment of proteins encoded by Nipi genes in selected protein complexes. The components of several known complexes are shown, namely, clockwise from top left, with the complex ID(s) in brackets (Additional file 10: Table S9), CCR4-NOT (YChrMod1004), eukaryotic translation initiation factor 2B complex (195; AMMC1231), mitochondrial ETC (218; AMMC1876), 66S pre-ribosomal particles (169; AMMC1267), mitochondrial complex I (65; AMMC1203), and in the center, mitochondrial oxoglutarate dehydrogenase complex (OGDC; YPC_CON1369). Proteins in dark green correspond to members of the final set of 338 Nipi genes, those in light green and orange to the presumed targets of clones that passed the first round of selection or that gave a single positive result in the first round, respectively. The clone that potentially knocks down lpd-5 (corresponding protein in red) abrogated nlp-29p::gfp expression but had a marked effect on development and reduced col-12p::dsRed expression. The remaining proteins are shown in blue