Author response image 4. The cell size of S. cerevisiae cells in response to low pH adjustment was measured over time with an RT-DC setup.
Cells were loaded into the microfluidic chip of the RT-DC device immediately after exposure to phosphate buffer of pH 6 containing 2 mM DNP and 2% glucose. The median diameter of more than 530 cells was measured for each time-point. We observed a small but significant decrease in cell size over a time span of 30 min. A two sided t-test shows that the slope differs significantly from zero.