Author response image 1. Summary of experiments showing that the cell wall was completely removed in our spheroplasting procedure.
(A) A rod-shaped spheroplasted fission yeast cell rounds up when media is added. (B) Acidified control and spheroplasted cells were treated with calcofluor white to stain the cell wall. Note the difference in signal intensity. The graphs on the right show the average calcofluor intensity of ten cells measured through a line scan. The location of the line scan is indicated for two representative cells with a red dashed line. (C) Stills of a time-lapse experiment where acidified rodshaped cells can be seen loosing their cell wall (yellow arrows). Also see corresponding Movie R1