Fig. 2.

Nos1ap^Tg; Mlc2v-cre (Tg⁺;cre⁺) mice from two transgenic lines overexpress Nos1ap transcript in left ventricles. a Box-and-Whisker plots showing relative expression levels for Nos1ap transcript in left ventricles from Tg⁺;cre⁺ (n = 10), Tg⁺;cre⁻ (conditional transgene only, n = 3) and wild type control littermates (Tg⁻;cre⁻, n = 10) mice derived from transgenic founder 112. Expression of Nos1ap transcript was higher in Tg⁺;cre⁺ mice as compared to Tg⁻;cre⁻ mice, t(18) = 5.77, p <0.001 and expression of Nos1ap transcript was not significantly different between Tg⁺;cre⁻ and Tg⁻;cre⁻mice, t(11) = 0.66, p = 0.52; b same as a, mice derived from transgenic founder 333 (Tg⁺;cre⁺ n = 7, Tg⁺;cre⁻ n = 5, Tg⁻;cre⁻ n = 7). Expression of Nos1ap transcript was higher in Tg⁺;cre⁺ mice as compared to Tg⁻;cre⁻ mice, t(12) = 5.98, p <0.001 and expression of Nos1ap transcript was not significantly different between Tg⁺;cre⁻ and Tg⁻;cre⁻ mice, t(10) = 0.07, p = 0.94; c Same as a, mice derived from transgenic founder 314 (Tg⁺;cre⁺ n = 8, Tg⁻;cre⁻ n = 7). No significant difference in expression level was observed between the two groups of mice derived from transgenic founder 314, t(13) = 0.22, p = 0.83