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. Author manuscript; available in PMC: 2016 Oct 8.
Published in final edited form as: Science. 2016 Apr 7;352(6282):231–235. doi: 10.1126/science.aad4017

Figure 2. Expression and activity of LbNOX in human cells.

Figure 2

(A) Western blot of LbNOX and mitoLbNOX in HeLa cells after 24-hour induction with water or doxycycline (300 ng/ml). Representative gel from one of three independent experiments. (B) Subcellular localization of LbNOX and mitoLbNOX in HeLa cells determined by cell fractionation. LRPPRC is a mitochondrial marker and Actin is a cytosolic marker. Representative gel from one of three independent experiments. (C) Subcellular localization of LbNOX and mitoLbNOX in HeLa cells determined using fluorescence microscopy. Tomm20 is a marker of mitochondria. (D) Effect of LbNOX and mitoLbNOX expression in HeLa cells on basal, piericidin-resistant and antimycin-resistant oxygen consumption measured with a XF24 extracellular flux analyzer. Mean ± S.E., n=3 independent experiments.