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. 2015 Oct;1(1):a000257. doi: 10.1101/mcs.a000257

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© 2015 Petrovski et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits reuse and redistribution, except for commercial purposes, provided that the original author and source are credited.

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Figure 1. — Western blot analysis using crude membrane fractions of HEK293 expressing the empty vector, WT-SLC52A2-Flag (WT), Q270*-SLC52A2-Flag (Q270X), and L339P-SLC52A2-Flag(L339P) variants. The blot was probed using anti-Flag and β-actin antibodies. The arrow highlights the location of the faint band of the truncated protein due to Q270* mutation at ∼30 kDa. The L339P mutation results in decreased protein expression. The functional effect of the L339P missense has been previously described (Foley et al. 2014).