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. Author manuscript; available in PMC: 2016 Jul 14.
Published in final edited form as: Nature. 2016 Jan 14;529(7585):212–215. doi: 10.1038/nature16504

Figure 3. Reintroduction of lost taxa and a high-MAC diet restores microbiota diversity and composition.

Figure 3

a, Schematic of fecal transplant mouse experiment. b, Principal coordinate analysis of UniFrac distance for 16S rRNA amplicon profiles from fecal samples collected from fourth generation control mice on a high-MAC diet (green, n=6), fourth generation, diet-switching mice that received a fecal transplant (red, n=3), or did not (blue, n=3). c, Microbiota diversity as measured by Shannon index observed in the microbiota of mice that received a fecal transplant (red, n=3) or did not (blue, n=3). A green circle denotes the number of OTUs observed in fourth generation control mice consuming a high-MAC diet (n=6). Error bars are shown as s.e.m. d, Heat map of abundance of high-confidence OTUs (number of sequencing reads) from fourth generation diet-switching mice (n=3) three to 14 days after FMT (fecal microbiota transplant) and no FMT controls (n=3); taxonomic assignment is indicated at the top of each column (Bacteroidetes, green; Firmicutes, orange; other, grey). FMT donor (fourth generation control mice, n=5) and fourth generation diet-switching mice (n=5) four weeks after consuming high-MAC diet are also shown.