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. Author manuscript; available in PMC: 2017 Jan 22.

Published in final edited form as: Circ Res. 2015 Nov 12;118(2):203–215. doi: 10.1161/CIRCRESAHA.115.307399

A. Ionic currents during triggered APs illustrating the effects of varying the fraction of nifedipine broken down by applying UV pulses of different energies. Similar results were obtained in 6 hearts. B. Ca²⁺ transients, measured with X-Rhod-5F AM, recorded simultaneously at the same location where the ionic currents (panel A) were obtained. The colors of the fluorescent traces correspond to the LPP currents. C. Relationship between Ca²⁺ transients amplitude and i_early. There is a direct relationship between intracellular Ca²⁺ and the Ca²⁺ influx (n=3 hearts). D. Ionic current traces obtained with different flash energies in hearts not perfused with exogenous Ca²⁺ indicators. E. Fluence dependency of i_late (blue) and i_early (black), both current components saturate for high fluences indicating that at these energies all nifedipine molecules were locally broken down. F. Nonlinear relationship between i_late and i_early (n=15 hearts). G. Time courses of normalized early currents recorded at diferent fluences. H. Relaxation time constants of the early currents as function of the photolysis fluence. I. Relaxation time constants of the early currents as function of the peak early current amplitude. (n= 4 hearts).

A. Ionic currents during triggered APs illustrating the effects of varying the fraction of nifedipine broken down by applying UV pulses of different energies. Similar results were obtained in 6 hearts. B. Ca²⁺ transients, measured with X-Rhod-5F AM, recorded simultaneously at the same location where the ionic currents (panel A) were obtained. The colors of the fluorescent traces correspond to the LPP currents. C. Relationship between Ca²⁺ transients amplitude and i_early. There is a direct relationship between intracellular Ca²⁺ and the Ca²⁺ influx (n=3 hearts). D. Ionic current traces obtained with different flash energies in hearts not perfused with exogenous Ca²⁺ indicators. E. Fluence dependency of i_late (blue) and i_early (black), both current components saturate for high fluences indicating that at these energies all nifedipine molecules were locally broken down. F. Nonlinear relationship between i_late and i_early (n=15 hearts). G. Time courses of normalized early currents recorded at diferent fluences. H. Relaxation time constants of the early currents as function of the photolysis fluence. I. Relaxation time constants of the early currents as function of the peak early current amplitude. (n= 4 hearts).