Table 2.
Redox sensitive targets with relative quantification of oxidative state of susceptible Cys residues and relative protein abundance, proteins in bold also change in abundance.
| Accession | Protein | Adult Vs. aged | (-)10log P | Redox Cys | Adult Red/Ox | Aged Red/Ox |
|---|---|---|---|---|---|---|
| P68254 | 14-3-3 protein theta (Ywaq) | 1.00:1.59 | 21.52 | Cys134 | 4.38 | 4.12 |
| P63101 | 14-3-3 protein zeta/delta (Ywhaz) | 1.00:1.34 | 11.31 | Cys25 | 3.64 | 3.16 |
| Cys94 | 4.81 | 4.41 | ||||
| P68134 | Actin, alpha skeletal muscle (Acta1) | 1.00:0.4 | 59.6 | Cys287 | 7.53 | 3.90 |
| P60710 | Actin, cytoplasmic 1 (Actb) | 1.00:1.69 | 10.82 | Cys257 | 12.96 | 10.24 |
| P17183/2 | Gamma-enolase (Eno1)/Alpha-enolase (Eno1) | 1.00:1.41 | 8.84 | Cys399 | 3.09 | 2.58 |
| P07724 | Albumin serum (Alb) | 1.00:1.78 | 45.23 | Cys58 | 0.82 | 1.34 |
| Cys77 | 0.02 | 0.03 | ||||
| Cys289 | 0.02 | 0.02 | ||||
| Cys500/501 | 0.03 | 0.01 | ||||
| P14824 | Annexin A6 (Anxa6) | 1.00:1.14 | 2.99 | Cys114 | 9.54 | 8.44 |
| Q61878 | Bone marrow proteoglycan (Prg2) | 0.00:0.100 | 36.64 | Cys198/202 | 3.15 | 2.44 |
| P10605 | Cathepsin B (Ctsb) | 1.00:8.49 | 30.49 | Cys105/108 | 0.15 | 0.13 |
| Q04447 | Creatine kinase B-type (Ckb) | 1.00:0.68 | 0.06 | Cys254 | 1.25 | 1.16 |
| Q9CZ13 | Cytochrome b-c1 complex subunit 1, mitochondrial (Uqcrc1) | 1.00:0.46 | 16.83 | Cys380 | 1.57 | 1.99 |
| Q80UW2 | F-box only protein 2 (Fbxo2) | 1.00:0.94 | 1.88 | Cys75 | 6.42 | 5.96 |
| Cys79 | 0.10 | 0.11 | ||||
| P16045 | Galectin-1 (Lgals1) | 1.00:1.97 | 14.51 | Cys61 | 4.14 | 3.28 |
| Q6PAC1 | Gelsolin, isoform CRA_c (Gsn) | 1.00:1.38 | 17.23 | Cys280 | 7.92 | 7.28 |
| Cys670 | 130.47 | 135.52 | ||||
| P16858 | Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | 1.00:0.96 | 1.23 | Cys245 | 6.02 | 5.46 |
| P62874 | Guanine nucleotide-binding protein subunit beta-1 (Gnb1) | 1.00:0.89 | 2.45 | Cys148/149 | 22.75 | 11.48 |
| P06151 | l-lactate dehydrogenase A chain (Ldha) | 1.00:1.54 | 11.12 | Cys163 | 2.13 | 1.90 |
| P16125 | l-lactate dehydrogenase B chain (Ldhb) | 1.00:1.65 | 9.36 | Cys163 | 2.13 | 1.90 |
| P97457 | Myosin regulatory light chain 2, skeletal muscle isoform (Mylpf) | 1.00:0.32 | 31.83 | Cys128 | 2.93 | 1.79 |
| F7A3A1 | Neurofilament heavy polypeptide (Nefh) | 1.00:0.81 | 9.84 | Cys225 | 4.26 | 4.16 |
| Cys246 | 1.91 | 1.94 | ||||
| Cys263 | 9.44 | 7.21 | ||||
| Cys409 | 6.11 | 6.98 | ||||
| P08551 | Neurofilament light polypeptide (Nefl) | 1.00:0.80 | 10.71 | Cys323 | 5.34 | 2.88 |
| Q99ME9 | Nucleolar GTP-binding protein 1 (Gtpbp4) | 1.00:1.67 | 12.49 | Cys336 | 13.15 | 15.35 |
| E9QQ57 | Periaxin (Prx) | 1.00:1.13 | 5.08 | Cys86 | 3.56 | 4.06 |
| G5E846 | Peripherin (Prph) | 1.00:0.74 | 13.04 | Cys148 | 2.85 | 2.39 |
| Cys328 | 3.45 | 2.77 | ||||
| P17742 | Peptidyl-prolyl cis-trans isomerase A (Ppia) | 1.00:1.11 | 2.26 | Cys62 | 1.85 | 1.81 |
| O08709 | Peroxiredoxin 6 (Prdx6 ) | 1.00:1.54 | 21.46 | Cys47 | 1.36 | 1.05 |
| Q61233 | Plastin 2 (Lcp1) | 1.00:3.20 | 48.17 | Cys336 | 0.22 | 0.42 |
| P52480 | Pyruvate kinase isozymes M1/M2 (Pkm2) | 1.00:0.56 | 28.18 | Cys49 | 4.20 | 3.90 |
| P50396 | Rab GDP dissociation inhibitor alpha (Gdi1 ) | 1.00:1.28 | 7.7 | Cys202 | 4.40 | 3.80 |
| Q99PT1 | Rho GDP-dissociation inhibitor 1 (Arhgdia) | 1.00:1.72 | 25.46 | Cys79 | 10.32 | 12.91 |
| P07759 | Serine protease inhibitor A3K (Serpina3k) | 1.00:1.21 | 2.05 | Cys260 | 4.13 | 5.47 |
| P16546 | Spectrin alpha chain brain (Sptan1) | 1.00:0.88 | 1.24 | Cys956 | 4.20 | 3.90 |
| Cys1454 | 3.56 | 2.90 | ||||
| Q8VDN2 | Sodium/potassium-transporting ATPase subunit alpha−1 (Atp1a1) | 1.00:0.96 | 1.21 | Cys249 | 3.52 | 3.37 |
| Q62465 | Synaptic vesicle membrane protein VAT−1 homologue (Vat1) | 1.00:2.31 | 32.94 | Cys99 | 0.84 | 0.98 |
| H7BXC3 | Triosephosphate isomerase (Tpi1) | 1.00:1.49 | 8.47 | Cys136 | 3.77 | 2.72 |
| P68369/8 | Tubulin alpha−1 A chain (Tuba1a)/Tubulin alpha−4 A chain (Tuba4a) | 1.00:0.78 | 7.26 | Cys315/316 | 3.85 | 2.02 |
| Cys376 | 1.16 | 1.05 | ||||
| Cys303 | 5.50 | 5.40 | ||||
| Q9R0P9 | Ubiquitin carboxyl-terminal hydrolase isozyme L1 (Uchl1) | 1.00:1.14 | 2.75 | Cys152 | 6.00 | 5.78 |
| P20152 | Vimentin OS (Vim) | 1.00:1.67 | 35.87 | Cys328 | 3.91 | 3.59 |