Extended Data Figure 9. m⁶A modification promotes cap-independent translation.

a, Fluc reporter mRNAs with or without 5′UTR was synthesized in the absence or presence of m⁶A. The transfected MEFs were incubation in the presence of 5 μg/ml ActD. At the indicated times, mRNA levels were determined by qPCR. Error bars, mean ± s.e.m.; n=3, biological replicates. b, Fluc reporter mRNAs with or without Hsp70 5′UTR was synthesized in the absence of presence of m⁶A, followed by addition of a non-functional cap analog A_pppG. Fluc activity in transfected MEF cells was recorded using real-time luminometry. c, Constructs expressing Fluc reporter bearing 5′UTR from Hsc70 or Hsp105 are depicted on the top. Fluc activities in transfected MEF cells were quantified and normalized to the control containing normal A. Error bars, mean ± s.e.m.; *p < 0.05, unpaired two-tailed t-test; n=3, biological replicates.