Figure 1. C-Myc asymmetrically segregates to the proximal daughter in activated CD8 T lymphocytes.

(A) Mean fluorescent intensities (MFI) of c-Myc-GFP in negative (wt cells; gold histogram), CD8^low (gray histogram), and CD8^high (green histogram) cells in the first (left panel) and second (right panel) divisions. Representative of four independent experiments. (B) Representative image of conjoined daughter c-Myc-GFP CD8 T cells (antibody-coated plates) fixed and stained for beta tubulin (blue) and CD8 (red). (C) Quantification of asymmetry based on fluorescent intensities of CD8 (difference/total; x axis) and c-Myc-GFP (difference/total; y axis). 88.9% bright in same daughter (p=0.0004 Two-Tailed Binomial Test); r²=0.6159, p<0.0001 Linear Regression. Compiled from four independent experiments; each point represents a conjoined daughter pair. (D–E) Representative image and quantification of asymmetry of conjoined daughter OT-I cells co-cultured with BMDCs. 86.2% both bright in proximal daughter (x²=58.31, DF=3, p<0.0001 Chi-Square Goodness of Fit); r²=0.3939, p=0.0003; Linear Regression (F–H) Quantifications of asymmetry (difference/total) of ACD markers Numb 100% both bright in proximal daughter (x²=33, DF=3, p<0.0001 Chi-Square Goodness of Fit); r²=0.5738, p=0.0069 Linear Regression (F), Scribble 100% both bright in proximal daughter (x²=30, DF=3, p<0.0001 Chi-Square Goodness of Fit); r²=0.01436, p=0.7416; Linear Regression (G), and PKC zeta 85% PKC zeta bright in distal daughter, c-Myc bright in proximal daughter (x²=26.8, DF=3, p<0.0001 Chi-Square Goodness of Fit); r²=0.0009705, p=0.8963 Linear Regression (H). (I–J) Representative image and quantification of c-Myc fluorescence of dividing OT-I T cells in spleen sections of animals infected with Listeria-OVA, p=0.0077; Paired Student’s T. Compiled from two independent experiments