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. 2016 Apr 29;11(4):e0152723. doi: 10.1371/journal.pone.0152723

Fig 3. ITC data.

Fig 3

Titrations of d-Phe (a) and d-Trp ((b), (c)) into a cell containing 5 μM MtuDAH7PS tetramer, corrected for heats of dilution. (a) d-Phe (syringe concentration 3 mM) titration into a cell containing MtuDAH7PS: fit of a one-site model to the data gives a Kd of 93 ± 5 μM. (b) d-Trp (syringe concentration 12 mM) titration into a cell containing MtuDAH7PS: fit of a one-site model to the data gives a Kd of 700 ± 150 μM. (c) d-Trp (syringe concentration 12 mM) titration into a cell containing MtuDAH7PS in the presence of 200 μM l-Phe: fit of a sequential two-site model to the data gives dissociation constants of 550 ± 60 μM and 1500 ± 330 μM.