Fig 4. Levels of pre-therapy IFN-γ-or IL-2 secreting T cells in relation to the evolution to T. cruzi-specific humoral responses in Chagas disease patients treated with benznidazole.

Treated subjects were grouped as those with stable or declining T. cruzi-specific antibodies post-treatment as measured by conventional serological tests and multiplex assays. Each dot represents the mean IFN-γ (A) and IL-2 (B) spot number of triplicate wells for each patient sample assessed. Spot counts with media alone were subtracted from T. cruzi–antigen stimulated spot numbers. Horizontal lines depict median values. Comparisons between groups were performed using the Mann-Whitney U test. P < 0.05 was considered as statistically significant.