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. 2016 May;89(5):521–540. doi: 10.1124/mol.115.103044

Fig. 2.

Fig. 2.

NDRG1 expression inhibits phosphorylation of FAK and paxillin using HT29 (A) and DU145 (B) cells. Immunoblotting was conducted to examine NDRG1 expression, phosphorylation of FAK (Tyr397, Tyr567/7, and Tyr925), total FAK, phosphorylation of paxillin (Tyr118), and total paxillin using NDRG1 overexpressing and silencing (sh-NDRG1) models and their respective controls in both cell types. Immunoblots shown are representative of three independent experiments. Densitometry for NDRG1 and total FAK/paxillin expression are expressed relative to the loading control, β-actin, whereas the phosphorylation levels for FAK and paxillin are displayed both relative to β-actin and as a ratio of their respective total protein levels, as shown on separate graphs. Densitometry data are shown as mean ± S.D. relative to the respective vector control or sh-Control cells as appropriate (3–5 experiments). *P < 0.05; **P < 0.01; ***P < 0.001.