Fig. 5.

NDRG1 mediates paxillin phosphorylation via a FAK-dependent mechanism as demonstrated using FAK siRNA in HT29 and DU145 cells. The sh-Control and sh-NDRG1 HT29 (A) and DU145 (B) cells were incubated with FAK-specific siRNA (si-FAK) or negative control siRNA (si-Con). Immunoblots shown are representative of three independent experiments. Densitometry for NDRG1 and total FAK/paxillin expression are expressed relative to the loading control, β-actin, whereas the phosphorylation levels for FAK and paxillin are displayed both relative to β-actin and as a ratio of their respective total protein levels as shown on separate graphs. Densitometry data are shown as mean ± S.D. relative to si-Con for both sh-NDRG1 and sh-Control cells (3–5 experiments). *P < 0.05; **P < 0.01; ***P < 0.001.