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. 2016 May;89(5):575–584. doi: 10.1124/mol.116.103341

Fig. 2.

Fig. 2.

ACh concentration-response curves of (α4)3(β2)2 and (α4)2(β2)3 nAChRs. Xenopus oocytes injected with nAChR subunit mRNAs mixed at a ratio of 9α4:1β2 [▴, (α4)3(β2)2 nAChR] or 1α4:9β2 [⬤, (α4)2(β2)3 nAChR] were voltage clamped at −50 mV, and currents elicited by 10-second applications of increasing concentrations of ACh were recorded. (A) Representative traces of ACh-induced current responses of (α4)3(β2)2 and (α4)2(β2)3 nAChRs. For each concentration, the peak currents were normalized to the peak current elicited by 1 mM ACh, and averages (±S.E.) of replicas from multiple oocytes (▴, 4; ⬤, 6) were plotted (B) and fit to a three-parameter Hill equation (Materials and Methods, eq. 1). ACh EC50 values/Hill coefficients for (α4)3(β2)2 and (α4)2(β2)3 nAChRs were 160 ± 24 μM/0.9 ± 0.1 and 1.1 ± 0.04 μM/1.2±0.04, respectively.