Intragastric administration of GL fails to prevent APAP-induced hepatotoxicity. (A) H&E-stained liver sections 24 hours after APAP challenge. Original magnification, 20×; black scale bar, 50 µm. (B, C) Serum ALT and AST levels. (D, E) GL and GA levels in serum and liver. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 (po) + APAP, GL50 mg/kg (oral)/APAP-treated mice; GL100 (po) + APAP, GL100 mg/kg (oral)/APAP-treated mice; GL50 (ip) + APAP, GL50 mg/kg (i.p.)/APAP-treated mice. Data are expressed as mean ± S.E.M., n = 4–5 in each group. ###P < 0.001 versus control mice; **P < 0.01 versus APAP-overdosed mice.