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. 2016 May;357(2):248–257. doi: 10.1124/jpet.115.229542

Fig. 7.

Fig. 7.

Fluorescence intensity of POMC mRNA during forced abstinence. (A) POMC mRNA, in red, detected by RNAscope. Brains were harvested and snap-frozen 30 minutes following the last experimental session on day 23. Six 0.14-μm coronal sections were taken from each animal (n = 5 for all groups, total of 30 sections per group) and analyzed for total POMC mRNA fluorescence between –2 mm and –3.5 mm bregma. 3V, third ventricle; scale bar 100 μm. (B) Fluorescence intensity of POMC mRNA, expressed as mean ± S.E.M. Treatment had a significant effect on POMC mRNA intensity P = 0.0004. Bonferroni post-hoc analysis revealed a significant difference between the cocaine plus vehicle and the cocaine plus 3 mg/kg l-THP and 0.1 mg/kg LDN group. **P < 0.01, between cocaine plus 3 mg/kg l-THP and cocaine plus 3 mg/kg l-THP and 0.1 mg/kg LDN. *P < 0.01.