Fig. 1.

Effect of NEP inhibitor treatments on castor oil–induced experimental diarrhea. Rats were given a single oral treatment of vehicle (10:10:80 ethanol/kolliphor EL/water) or NEP inhibitor 30 minutes before castor oil administration as described in Materials and Methods. (A–C) Time course of cumulative stool weight measured in independent studies of racecadotril and other indicated NEP inhibitors. (D and F) Total area under the time course curves (AUC) calculated for each treatment from the stool accumulation data. Data are expressed as the mean ± S.E.M. (n = 10/group; owing to gavage injury, one animal was terminated early from both the vehicle and racecadotril groups shown in (A); these were excluded from the analysis). *P < 0.05 versus vehicle. ^#P < 0.05 versus racecadotril (two-tailed T test).