Fig. 5.

NEP inhibitor treatments do not delay intestinal transit. (A) Effects of loperamide and sacubitril treatments on gastrointestinal transit as evaluated using the charcoal meal test in rats. (B) Effects of loperamide, racecadotril, and candoxatril treatments on gastrointestinal transit in the same model. Animals received test agents 30 minutes before administration of the charcoal meal and were sacrificed 20 minutes later. Vehicle was 5% ethanol in water. The racecadotril formulation also contained 0.2% Tween 20 for solubility. Data are expressed as the mean ± S.E.M. of values from five rats. *P < 0.05 versus vehicle.