Skip to main content
. Author manuscript; available in PMC: 2016 Apr 29.
Published in final edited form as: Dev Biol. 2015 Jan 7;399(2):218–225. doi: 10.1016/j.ydbio.2014.12.032

Fig. 4.

Fig. 4

Ets1/2 and Elk1/3/4 have distinct larval phenotypes. (A–B) Control and FGF-inhibited larvae. (A) Control larvae possess two pigmented cells within the sensory vesicle. (B) ZicL > dnFGFR results in complete loss of pigmented cells in the majority of larvae. (C–D) Misexpression of Elk1/3/4 fusion proteins. (C) ZicL > Elk:VP64 larvae contain 2 pigmented cells, comparable to controls. (D) ZicL > Elk:WRPW results in fewer pigmented cells relative to controls, similar to ZicL > dnFGFR. (E, F) Misexpression of Ets fusion proteins. (E) ZicL > Ets:VP16 is sufficient to produce supernumerary pigmented cells, whereas ZicL > Ets:WRPW results in loss of pigmentation (F), as previously described. (G, H) Larvae electroporated with Tyrp1a > LacZ (G) or Tyrp1a > Six3/6 (H). Misexpression of Six3/6 in the pigmented cell lineage is sufficient to abolish pigmentation. Scale bars = 50 µm.